Phenolyimolekyuli edlala indima ebalulekileyo kwiikhemikhali ezininzi kwaye isetyenziswa kuluhlu olubanzi lwezicelo zemizi-mveliso.Ke ngoko, kubalulekile ukuba ube nendlela ethembekileyo yokuchonga i-phenol kwiisampulu ezahlukeneyo.Kweli nqaku, siza kuphonononga iindlela ezahlukeneyo ezikhoyo zokuchonga i-phenol, izibonelelo zabo kunye nokungalunganga, kunye nokubaluleka kokuchongwa kwe-phenol kubomi bemihla ngemihla nakwishishini.

1. IChromatography yerhasi (GC)

Ichromatography yegesi yindlela esetyenziswa ngokubanzi yohlalutyo lokuchonga i-phenol.Kule ndlela, isampuli ijojowe kwikholamu egcwele isigaba sokumisa.Isigaba esihambayo sihamba emva kwekholamu, sihlula amacandelo ngamnye wesampuli.Ukwahlula kusekelwe kwi-solubility ehambelanayo yamacandelo kwizigaba ezimileyo kunye nezihambayo.

Izinto eziluncedo: I-GC inobuntununtunu kakhulu, ithe ngqo, kwaye iyakhawuleza.Iyakwazi ukubona ukugxila okuphantsi kwephenol.

Ukungalungi: I-GC ifuna abasebenzi abaqeqeshwe kakhulu kunye nezixhobo ezibiza kakhulu, okwenza ukuba ingalungeli uvavanyo lwasendle.

2. I-Liquid Chromatography (LC)

Ichromatography yolwelo iyafana nechromatography yerhasi, kodwa isigaba esimileyo sipakishwe kwikholamu endaweni yokuba sigqunywe kwinkxaso emileyo.I-LC idla ngokusetyenziselwa ukwahlula iimolekyuli ezinkulu, ezinjengeeproteni kunye neepeptides.

Izinto eziluncedo: I-LC inobuchule obuphezulu bokwahlula kwaye inokuphatha iimolekyuli ezinkulu.

Ukungalungi: I-LC ayikhathali kangako kune-GC kwaye ifuna ixesha elingakumbi lokufumana iziphumo.

3. I-Spectroscopy

I-Spectroscopy yindlela engonakalisiyo ebandakanya ukulinganisa ukufunxwa okanye ukukhutshwa kwemitha ye-athomu okanye iimolekyuli.Kwimeko ye-phenol, i-infrared spectroscopy kunye ne-nuclear magnetic resonance (NMR) spectroscopy ziqhele ukusetyenziswa.I-infrared spectroscopy ilinganisa ukufunxwa kwemitha ye-infrared ngeeathom, ngelixa i-NMR spectroscopy ilinganisa ukufunxwa kwemitha ye-radiofrequency yi-nuclei ye-athomu.

Iingenelo: I-Spectroscopy icace kakhulu kwaye inokunika ulwazi oluneenkcukacha malunga nokwakheka kweemolekyuli.

Iingxaki: I<em>Spectroscopy idla ngokufuna izixhobo ezibiza imali eninzi yaye inokutya ixesha.

4. Iindlela zoMbala

Iindlela ze-colorimetric zibandakanya ukuphendula isampuli kunye ne-reagent ukuvelisa imveliso enemibala enokulinganiswa nge-spectrophotometrically.Enye indlela eqhelekileyo ye-colorimetric yokuchonga i-phenol ibandakanya ukusabela isampuli nge-4-aminoantipyrine phambi kwe-reagent yokudibanisa ukuvelisa imveliso enombala obomvu.Ubunzulu bombala buhambelana ngokuthe ngqo noxinaniso lwephenol kwisampulu.

Izinto eziluncedo: Iindlela zemibala zilula, azibizi, kwaye zingasetyenziselwa uvavanyo lwasendle.

Ukungalungi: Iindlela ze-colorimetric zinokuthi zingabonakali kwaye zingaboni zonke iintlobo ze-phenol.

5. Uvavanyo lwebhayoloji

Uvavanyo lwebhayolojiUkusebenzisa ukusabela okucacileyo kokuphila kwezinto eziphilayo ukubona ubukho, iimpawu, kunye nomxholo wezinto ekujoliswe kuzo.Ngokomzekelo, ezinye iibhaktheriya kunye negwele ziyakwazi ukuguqula i-phenol kwimveliso enemibala enokulinganiswa nge-spectrophotometrically.Olu vavanyo luthe ngqo kakhulu kodwa lusenokungabi novakalelo ekugxininiseni okuphantsi.

Izinto eziluncedo: Uvavanyo lwebhayoloji lungqale kakhulu kwaye lunokusetyenziswa ukuchonga iikhompawundi zenoveli.

Iingxaki: Uvavanyo lwebhayoloji lusenokungabi novelwano kwaye ludla ngokutya ixesha.