Phenolyimolekyuli edlala indima ebalulekileyo kwiikhemikhali ezininzi kwaye isetyenziswa kuluhlu olubanzi lwezicelo zemizi-mveliso. Ke ngoko, kubalulekile ukuba ube nendlela ethembekileyo yokuchonga i-phenol kwiisampulu ezahlukeneyo. Kweli nqaku, siza kuphonononga iindlela ezahlukeneyo ezikhoyo zokuchonga i-phenol, izibonelelo zabo kunye nokungalunganga, kunye nokubaluleka kokuchongwa kwe-phenol kubomi bemihla ngemihla nakwishishini.
1. IChromatography yerhasi (GC)
Ichromatography yegesi yindlela esetyenziswa ngokubanzi yohlalutyo lokuchonga i-phenol. Kule ndlela, isampuli ijojowe kwikholamu egcwele isigaba sokumisa. Isigaba esihambayo sihamba emva kwekholamu, sihlula amacandelo ngamnye wesampuli. Ukwahlula kusekelwe kwi-solubility ehambelanayo yamacandelo kwizigaba ezimileyo kunye nezihambayo.
Izinto eziluncedo: I-GC inobuntununtunu kakhulu, ithe ngqo, kwaye iyakhawuleza. Iyakwazi ukubona ukugxila okuphantsi kwephenol.
Ukungalungi: I-GC ifuna abasebenzi abaqeqeshwe kakhulu kunye nezixhobo ezibiza kakhulu, okwenza ukuba ingalungeli uvavanyo lwasendle.
2. I-Liquid Chromatography (LC)
Ichromatography yolwelo iyafana nechromatography yerhasi, kodwa isigaba esimileyo sipakishwe kwikholamu endaweni yokuba sigqunywe kwinkxaso emileyo. I-LC idla ngokusetyenziselwa ukwahlula iimolekyuli ezinkulu, ezinjengeeproteni kunye neepeptides.
Izinto eziluncedo: I-LC inobuchule obuphezulu bokwahlula kwaye inokuphatha iimolekyuli ezinkulu.
Ukungalungi: I-LC ayikhathali kangako kune-GC kwaye ifuna ixesha elingakumbi lokufumana iziphumo.
3. I-Spectroscopy
I-Spectroscopy yindlela engonakalisiyo ebandakanya ukulinganisa ukufunxwa okanye ukukhutshwa kwemitha ye-athomu okanye iimolekyuli. Kwimeko ye-phenol, i-infrared spectroscopy kunye ne-nuclear magnetic resonance (NMR) spectroscopy ziqhele ukusetyenziswa. I-infrared spectroscopy ilinganisa ukufunxwa kwemitha ye-infrared ngeeathom, ngelixa i-NMR spectroscopy ilinganisa ukufunxwa kwemitha ye-radiofrequency yi-nuclei ye-athomu.
Iingenelo: I-Spectroscopy icace kakhulu kwaye inokunika ulwazi oluneenkcukacha malunga nokwakheka kweemolekyuli.
Iingxaki: ISpectroscopy idla ngokufuna izixhobo ezibiza imali eninzi yaye inokutya ixesha.
4. Iindlela zoMbala
Iindlela ze-colorimetric zibandakanya ukuphendula isampuli kunye ne-reagent ukuvelisa imveliso enemibala enokulinganiswa nge-spectrophotometrically. Enye indlela eqhelekileyo ye-colorimetric yokuchonga i-phenol ibandakanya ukusabela isampuli nge-4-aminoantipyrine phambi kwe-reagent yokudibanisa ukuvelisa imveliso enombala obomvu. Ubunzulu bombala buhambelana ngokuthe ngqo noxinaniso lwephenol kwisampulu.
Izinto eziluncedo: Iindlela zemibala zilula, azibizi, kwaye zingasetyenziselwa uvavanyo lwasendle.
Ukungalungi: Iindlela ze-colorimetric zinokuthi zingabonakali kwaye zingaboni zonke iintlobo ze-phenol.
5. Uvavanyo lwebhayoloji
Uvavanyo lwebhayolojiUkusebenzisa ukusabela okucacileyo kokuphila kwezinto eziphilayo ukubona ubukho, iimpawu, kunye nomxholo wezinto ekujoliswe kuzo. Ngokomzekelo, ezinye iibhaktheriya kunye negwele ziyakwazi ukuguqula i-phenol kwimveliso enemibala enokulinganiswa nge-spectrophotometrically. Olu vavanyo luthe ngqo kakhulu kodwa lusenokungabi novakalelo ekugxininiseni okuphantsi.
Izinto eziluncedo: Uvavanyo lwebhayoloji lungqale kakhulu kwaye lunokusetyenziswa ukuchonga iikhompawundi zenoveli.
Iingxaki: Uvavanyo lwebhayoloji lusenokungabi novelwano kwaye ludla ngokutya ixesha.
Ixesha lokuposa: Dec-12-2023
