PhenolYimolekyuli edlala indima ebalulekileyo ekuphenduleni kweekhemikhali kwaye isetyenziswa kuluhlu olubanzi lwezicelo zeshishini. Ke ngoko, kubalulekile ukuba nendlela ethembekileyo yokuchonga i-phenol kwiisampulu ezahlukeneyo. Kweli nqaku, siza kuphonononga iindlela ezahlukeneyo zokuchonga i-phenol, izibonelelo zabo kunye nezinto ezingalunganga, kunye nokubaluleka kokuchongwa kwe-phenol kubomi bemihla ngemihla.

1. I-chromatography (GC)

I-chromatography yegesi yindlela yokuhlalutya ngokubanzi yokuchonga i-phenol. Kule ndlela, isampulu igcinwe kwikholam ezaliswe linqanaba lesikhululo. Inqanaba leselula emva koko lihamba kwikholamu, ukwahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlulahlula-hlula isampulu. Ukwahlukana kusekwe kwi-smonity yezinto ezihambelana nezigaba ezikwisikhululo nezezibalo.

Izibonelelo: I-GC inovelwano kakhulu, okhethekileyo, kwaye ngokukhawuleza. Ingafumana ugxininiso olusezantsi lwe-phenol.

Izinto ezingalunganga: I-GC ifuna abasebenzi abaqeqeshiweyo kunye nezixhobo ezixabisa kakhulu, zenza ukuba zibe lula kuvavanyo lwensimu.

2. I-chromatography yolwelo (LC)

I-chromatography ye-chromatography ifana ne-chromatography yegesi, kodwa iSigaba sokuma sipakishwe kwikholamu endaweni yokuba idityaniswe kwinkxaso yesikhululo. I-LC iqhele ukusetyenziselwa ukwahlula iimolekyuli ezinkulu, ezinjengeeproteni kunye ne-peptides.

Izibonelelo: I-LC inobuchule obuphezulu kwaye unokujongana neemolekyuli ezinkulu.

Izinto ezingalunganga: I-LC ayibuthwala i-GC kwaye ifuna ixesha elingakumbi lokufumana iziphumo.

I-3. I-Spectudroscopy

I-SpecicroscopyOpcopy yindlela engeyiyo eyona nto ibandakanya ukulinganisa i-athomu okanye yokuphuma kweeatom okanye iimolekyuli. Kwimeko ye-phenol, i-infrared spectroscopy kunye ne-nuclearl resonance (i-NMR) i-Spectrascopy isetyenziswa. I-infrared spectroscoppy ilinganisa imitha ye-infrared ye-molecules, ngelixa i-NMR Spectudroscopy ilinganisa i-raderedraquety raction yi-nuclei ye-nuclei ye-nuclei.

Izibonelelo: I-Specctroscopy icacile kakhulu kwaye inokubonelela ngolwazi oluneenkcukacha malunga nolwakhiwo lweeMolekyuli.

Izinto ezingalunganga: I-Spectroscopy zihlala zifuna izixhobo ezithi zixabisa kwaye zinokuthatha ixesha.

I-4. Iindlela ze-lemilim

Iindlela zemidlalo yemibala zibandakanya ukuqalisa isampulu kunye nokuphinda isebenze ukuze kuveliswe imveliso enemibala enokuthi ilinganiswe i-spectramestrictricketcallrically. Indlela enye yezilwanyana eqhelekileyo yokuchonga i-phenol ibandakanya ukuphendula isampulu ene-4-ye-aminoantipyrine phambi komgangatho wokudibanisa kwakhona ukuvelisa imveliso ebomvu. Ubunzulu bombala buhambelana ngokuthe ngqo kuxinzelelo lwe-phenol kwisampulu.

Iinzuzo: Iindlela zeeMoto ezilula zilula, azibizi, kwaye zingasetyenziselwa uvavanyo lwensimu.

Izinto ezingalunganga: Iindlela ze-lemilim zisenokungakwazi ukuqina kwaye aziyi kufumanisa zonke iintlobo ze-phenol.

I-5. I-Assays yebhayoloji

I-Biological Asysusetion Reaction yezinto eziphilayo ukuze ufumane ubukho, iipropathi, kunye neziqulatho zezinto ekujoliswe kuzo. Umzekelo, ezinye iibhaktiriya kunye ne-neard zinokuguqula i-phenol kwimveliso enemibala enokulinganiswa nge-spectramestralrimest. Ezi ndawo ziqinisekileyo zicacile kakhulu kodwa zinokusilela ekubonakalisweni.

Izibonelelo: I-Assay yebhayiloji icacile kakhulu kwaye inokusetyenziselwa ukuchonga i-inoveli.

Izinto ezingalunganga: I-assays yebhayiloji isenokungafezekisi kwaye ihlala ichitha ixesha.